多组学揭示SGA胎盘潜在调控基因

Integrated proteomic and transcriptomic landscape of human placenta in small for gestational age infants  

小于胎龄儿胎盘的转录组与蛋白质组学的整合图谱

Authors: Heyue Jin , Xianyan Wang , Lingyu Li , Chen Rui , Hong Gan , Qunan Wang , Fangbiao Tao , Yumin Zhu.

Source: iScience. 

DOI: 10.1016/j.isci.2024.111423.

Abstract

Small for gestational age (SGA) infants affected by placental insufficiency are exposed to the risk of stillbirth and long-term complications. Based on RNA-seq and mass spectrometry, we identified dysregulated RNAs and proteins from the comparisons of SGA placental tissues and controls. We revealed two SGA-relevant co-expression modules (SRMs) that also significantly distinguished SGA from controls. Then we performed an integrated analysis of transcriptomic and proteomic profiles to trace their links to SGA as well as their significant correlations. For the core functional molecules we screened, we revealed their potential upstream regulators and validated them experimentally in an independent cohort. Overall, we pointed out insights into different molecular pathways for the pathological mechanisms of SGA and indicated potential target molecules that may be drivers of placental aberrations in the SGA infants.

Keywords: Developmental anatomy; Pregnancy; Proteomics; Transcriptomics.

摘要

胎盘功能不全导致小于胎龄儿（Small for gestational age，SGA）面临死产和长期并发症的风险。基于RNA-seq和质谱分析，我们从SGA胎盘组织和对照组织的比较中鉴定了异常调控的RNA和蛋白质。我们发现了两个与SGA相关的共表达模块（SGA-relevant co-expression modules，SRMs），能显著区分SGA与对照组。然后，我们对转录组和蛋白质组图谱进行了综合分析，以追踪它们与SGA的联系及其相关性。对于我们筛选出的核心功能分子，我们揭示了它们潜在的上游调节因子，并在一个独立队列中进行了实验验证。总体来说，我们阐明了SGA病理机制的不同分子通路，并指出了可能导致SGA婴儿胎盘变化的潜在目标基因。

关键词：发育解剖学；怀孕；蛋白质组学；转录组。