高浓度的尿雌马酚和雌马酚预测微生物种类可能与肝脏脂肪和其他肥胖标志物有利相关

Urinary equol and equol-predicting microbial species are favorably associated with body fat measures among Chinese adults

尿雌马酚和预测雌马酚的微生物种类与中国成年人的体脂测量值有利相关

Author: Honghua Zhang, Shaoxian Liang, Kewan Yin1, Yufeng Mo, Yamin Li, Yaning Lv, Hao Zhan5, Zhuang Zhang, Zhilei Shan, Zhiguo Guo, Shi Yin, Wanshui Yang

Source: Nutrition Research

DOI: 10.1016/j.tjnut.2024.07.024

ABSTRACT

Many studies have investigated the intake of dietary isoflavones in relation to obesity risk, while the association using objective biomarkers of isoflavones, particularly equol (a gut-derived metabolite of daidzein with greater bioavailability than other isoflavones) has been less studied. In addition, the associations between equol and gut microbiota profile at population level remain to be fully characterized. We aimed to identify equol-predicting microbial species and to investigate the associations of equol-predicting microbial species and urinary excretion of isoflavones including glycitein, genistein, daidzein, and equol with diverse obesity markers in free living-individuals. In this 1-year longitudinal study of 754 community-dwelling adults, urinary isoflavones, fecal microbiota, height, weight, and circumferences of waist and hip were measured at baseline and again after 1-year. Liver fat [indicated by controlled attenuation parameter (CAP)] and other body composition were also measured after 1-year. Linear models and linear mixed-effects models were used to analyze the associations for single measure and repeated measures, respectively. Among 305 participants (median age: 50 y, IQR: 37-59 y) including 138 men and 167 women, higher urinary excretion of equol was associated with lower CAP (β = -0.013, P < 0.001) and body fat mass (β= -0.014, P = 0.046). No association was found between any other urinary isoflavones and obesity markers (all P>0.05). We identified 21 bacterial genera whose relative abundance were positively associated with urinary equol concentrations (all P_{false discovery rate} < 0.05), and constructed an equol-predicting microbial score to reflect the overall equol-producing potential of host gut microbiota. This score was inversely associated with CAP (β = -0.040, P = 0.011).

Conclusions: High urinary equol concentrations and equol-predicting microbial species could be favorably associated with liver fat and other obesity markers.

摘要

许多研究调查了膳食异黄酮摄入量与肥胖风险的关系，而使用异黄酮的客观生物标志物与肥胖风险的关联研究较少，特别是雌马酚（黄豆苷元的肠道衍生代谢物，比其他异黄酮具有更高的生物利用度）与肥胖风险的关联研究更少。此外，雌马酚与肠道微生物群之间的关系在人群水平上的关联仍有待全面研究。我们旨在确定预测雌马酚的微生物种类，并在人群中研究尿液中异黄酮（包括黄豆黄素、染料木黄酮、黄豆苷元和雌马酚）和预测雌马酚的微生物种类与多种不同肥胖标志物的关联。在这项为期1年的纵向研究中，研究对象为754名社区居住的成年人。在基线时以及1年后，各测量了一次尿液中的异黄酮、粪便微生物群、身高、体重以及腰围和臀围等指标，共两次。在1年后测量了肝脏脂肪[通过控制衰减参数（CAP）表示]和其他身体成分指标。研究采用线性模型分析单次测量数据相关性，使用线性混合效应模型分析重复测量的数据相关性。在305名参与者（中位年龄：50岁，IQR，37-59岁）中，包括138名男性和167名女性，较高的尿液雌马酚与较低的CAP（β=-0.013，，P<0.001）和体脂量（β=-0.014，P=0.046）相关。未发现其他尿异黄酮与肥胖标志物之间存在关联（P均>0.05）。我们确定了21个相对丰度与尿雌马酚浓度呈正相关的细菌属（P_假发现率均<0.05），并构建了雌马酚预测微生物评分，以反映宿主肠道微生物群的整体雌马酚产生潜力。该评分与CAP呈负相关（β=-0.040，P=0.011）。高浓度的尿雌马酚和雌马酚预测微生物种类可能与肝脏脂肪和其他肥胖标志物有利相关。